Lab Tips and Resources - Das Lab

All new members of the Das lab should read and follow the documents below. Please approach Dr. Das if you have any questions, and view the list of links and papers related to our current research below.

Relevant Links

PomBase: comprehensive database for the fission yest Schizosaccharomyces pombe

PombeNet: Susan Forsburg's lab website

PubMed

Saccharomyces Genome Database (SGD)

ExPASy: SIB Bioinformatics Resource Portal

NCBI Blast

American Society for Cell Biology

ImageJ: Image Processing and Analysis

Boston College Department of Biology

National Institutes of Health

National Science Foundation

American Heart Association

American Cancer Society

Lymphoma and Leukemia Society

Life Science Research Foundation

Contractile-ring assembly in fission yeast cytokinesis: Recent advances and new perspectives. Lee IJ, Coffman VC, Wu JQ. Cytoskeleton (Hoboken). 2012 Oct;69(10):751-63.
Progress towards understanding the mechanism of cytokinesis in fission yeast. Pollard TD. Biochem Soc Trans. 2008 Jun;36(Pt 3):425-30.
Overview of fission yeast septation. Pérez P, Cortés JC, Martín-García R, Ribas JC. Cell Microbiol. 2016 Sep;18(9):1201-7.
The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12. Willet AH, McDonald NA, Bohnert KA, Baird MA, Allen JR, Davidson MW, Gould KL. J Cell Biol. 2015 Feb 16;208(4):391-9.
The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast. Ren L, Willet AH, Roberts-Galbraith RH, McDonald NA, Feoktistova A, Chen JS, Huang H, Guillen R, Boone C, Sidhu SS, Beckley JR, Gould KL. Mol Biol Cell. 2015 Jan 15;26(2):256-69.
Contractile ring stability in S. pombe depends on F-BAR protein Cdc15p and Bgs1p transport from the Golgi complex. Arasada R, Pollard TD. Cell Rep. 2014 Sep 11;8(5):1533-44.
The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis. Coffman VC, Sees JA, Kovar DR, Wu JQ. J Cell Biol. 2013 Oct 14;203(1):101-14.
Assembly and architecture of precursor nodes during fission yeast cytokinesis. Laporte D, Coffman VC, Lee IJ, Wu JQ. J Cell Biol. 2011 Mar 21;192(6):1005-21.
Mid1p/anillin and the septation initiation network orchestrate contractile ring assembly for cytokinesis. Hachet O, Simanis V. Genes Dev. 2008 Nov 15;22(22):3205-16.
Roles of the TRAPP-II Complex and the Exocyst in Membrane Deposition during Fission Yeast Cytokinesis. Wang N, Lee IJ, Rask G, Wu JQ. PLoS Biol. 2016 Apr 15;14(4):e1002437.
Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast. Huang J, Huang Y, Yu H, Subramanian D, Padmanabhan A, Thadani R, Tao Y, Tang X, Wedlich-Soldner R, Balasubramanian MK. J Cell Biol. 2012 Nov 26;199(5):831-47.
Contributions of turgor pressure, the contractile ring, and septum assembly to forces in cytokinesis in fission yeast. Proctor SA, Minc N, Boudaoud A, Chang F. Curr Biol. 2012 Sep 11;22(17):1601-8.
Assembly mechanism of the contractile ring for cytokinesis by fission yeast. Vavylonis D, Wu JQ, Hao S, O’Shaughnessy B, Pollard TD. Science. 2008 Jan 4;319(5859):97-100.
Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. Wu JQ, Sirotkin V, Kovar DR, Lord M, Beltzner CC, Kuhn JR, Pollard TD. J Cell Biol. 2006 Jul 31;174(3):391-402.
Counting cytokinesis proteins globally and locally in fission yeast. Wu JQ, Pollard TD. Science. 2005 Oct 14;310(5746):310-4.
Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. Wu JQ, Kuhn JR, Kovar DR, Pollard TD. Dev Cell. 2003 Nov;5(5):723-34.

Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth. Das M, Drake T, Wiley DJ, Buchwald P, Vavylonis D, Verde F. Science. 2012 Jul 13; 337(6091):239-43.
Negative feedback enhances robustness in the yeast polarity establishment circuit. Howell AS, Jin M, Wu CF, Zyla TR, Elston TC, Lew DJ. Cell. 2012 Apr 13; 149(2):322-33.
Scaffold-mediated gating of Cdc42 signaling flux. Rapali P, Mitteau R, Braun CR, Massoni-Laporte A, Ünlü C, Bataille L, Saint Arramon F, Gygi SP, McCusker D. Elife. 2017 Mar 17; 6. pii: e25257. doi: 10.7554/eLife.25257.
Phosphorylation-dependent inhibition of Cdc42 GEF Gef1 by 14-3-3 protein Rad24 spatially regulates Cdc42 GTPase activity and oscillatory dynamics during cell morphogenesis. Das M, Nuñez I, Rodriguez M, Wiley DJ, Rodriguez J, Sarkeshik A, Yates JR 3rd, Buchwald P, Verde F. Mol Biol Cell. 2015 Oct 1; 26(19):3520-34. doi: 10.1091/mbc.E15-02-0095.
Spontaneous cell polarization: Feedback control of Cdc42 GTPase breaks cellular symmetry. Martin SG. Bioessays. 2015 Nov; 37(11):1193-201. doi: 10.1002/bies.201500077. Epub 2015 Sep 4.
Polarity establishment requires localized activation of Cdc42. Woods B, Kuo CC, Wu CF, Zyla TR, Lew DJ. J Cell Biology. 2015 Oct 12; 211(1):19-26. doi: 10.1083/jcb.201506108.
Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking. Bendezú FO, Vincenzetti V, Vavylonis D, Wyss R, Vogel H, Martin SG. PLoS Biology. 2015 Apr 2; 13(4):e1002097.
Phosphatidylserine and GTPase activation control Cdc42 nanoclustering to counter dissipative diffusion. Sartorel E, Ünlü C, Jose M, Massoni-Laporte A, Meca J, Sibarita JB, McCusker D. Mol Biol Cell. 2018 Jun 1; 29(11):1299-1310. doi: 10.1091/mbc.E18-01-0051. Epub 2018 Apr 18.
Bud3 activates Cdc42 to establish a proper growth site in budding yeast. Kang PJ, Lee ME, Park HO. Journal of Cell Biology. 2014 Jul 7; 206(1):19-28. doi: 10.1083/jcb.201402040.
The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion. Kokkoris K, Gallo Castro D, Martin SG. Journal of Cell Science. 2014 May 1; 127(Pt 9):2005-16. doi: 10.1242/jcs.142174.
Inhibitory GEF phosphorylation provides negative feedback in the yeast polarity circuit. Kuo CC, Savage NS, Chen H, Wu CF, Zyla TR, Lew DJ. Current Biology. 2014 Mar 31; 24(7):753-9.
Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Gupta S, Mana-Capelli S, McLean JR, Chen CT, Ray S, Gould KL, McCollum D. Current Biology. 2013 Feb 18; 23(4):333-8. doi: 10.1016/j.cub.2013.01.014.
Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42. Smith SE, Rubinstein B, Mendes Pinto I, Slaughter BD, Unruh JR, Li R. Journal of Cell Biology. 2013 Sep 30;202(7):1091-106.
Non-uniform membrane diffusion enables steady-state cell polarization via vesicular trafficking. Slaughter BD, Unruh JR, Das A, Smith SE, Rubinstein B, Li R. Nature Communications. 2013; 4:1380.
Cdc42 explores the cell periphery for mate selection in fission yeast. Bendezú FO, Martin SG. Current Biology. 2013 Jan 7; 23(1):42-7.
Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast. Bendezú FO, Martin SG. Mol Biol Cell. 2011 Jan 1; 22(1):44-53. doi: 10.1091/mbc.E10-08-0720.
The conserved NDR kinase Orb6 controls polarized cell growth by spatial regulation of the small GTPase Cdc42. Das M, Wiley DJ, Chen X, Shah K, Verde F. Current Biology. 2009 Aug 11; 19(15):1314-9. doi: 10.1016/j.cub.2009.06.057.
Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth. Alvarez-Tabarés I, Grallert A, Ortiz JM, Hagan IM. Journal of Cell Science. 2007 Oct 15; 120(Pt 20):3589-601.
Tea4p links microtubule plus ends with the formin for3p in the establishment of cell polarity. Martin SG, McDonald WH, Yates JR 3rd, Chang F. Dev Cell. 2005 Apr; 8(4):479-91.
Gef1p, a new guanine nucleotide exchange factor for Cdc42p, regulates polarity in Schizosaccharomyces pombe. Coll PM, Trillo Y, Ametzazurra A, Perez P. Mol Biol Cell. 2003 Jan;14(1):313-23.

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