Publications: 2005 - present

Roberts, M.F., Khan, H.M., Goldstein, R., Reuter, N., and Gershenson, A. (2018) Search and subvert: minimalist bacterial phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes. Chem. Rev., in press.

Rosenberg, M.M., Redfield, A.G., Roberts, M.F., and Hedstrom, L. (2018) Dynamic characteristics of guanosine-5’-monophosphate reductase complexes revealed by high resolution 31P field-cycling NMR relaxometry. Biochemistry 57, 3146-3154.

Roberts, M.F. (2018) High-resolution applications of shuttle field-cycling NMR. Chapter 15 in Field Cycling NMR Relaxometry: Instrumentation, Model Theories and Applications (R. Kimich, editor), Royal Society Press.

Roberts, M.F. (2017) Defining a viral membrane-binding complex on an atomic level. Structure 25, 3-4.

Gradziel, C.S., Jordan, P.A., Jewel, D., Dufort, F.J., Miller, S.J., Chiles, T.C., and Roberts M.F. (2016) D-3-Deoxy-dioctanoylphosphatidylinositol induces cytotoxicity in human MCF-7 breast cancer cells via a mechanism that involves downregulation of the D-type cyclin-retinoblastoma pathway. Biochim. Biophys. Acta 1861, 1808-1815.

Rosenberg, M.M., Redfield, A.G., Roberts, M.F., and Hedstrom, L. (2016) Substrate and cofactor dynamics on guanosine monophosphate reductase probed by high resolution field cycling 31P NMR relaxometry. J. Biol. Chem. 291, 22988-22998.

Huang, Q., Gershenson, A., and Roberts, M.F. (2016) Recombinant broad-range phospholipase C from Listeria monocytogenes exhibits optimal activity at acidic pH. Biochim. Biophys. Acta 1864, 697-705.

Khan, H.M., He, T., Fuglebakk, E., Grauffel, C., Yang, B., Roberts, M.F., Gershenson, A., and Reuter, N. (2016) A role for weak electrostatic interactions in peripheral membrane protein binding. Biophys. J. 110, 1367-1378.

T. He, A. Gershenson, S. J. Eyles, Y.-J. Lee, W. R. Liu, J. Wang, J. Gao, and M. F. Roberts, “Fluorinated aromatic amino acids distinguish cation-π interactions from membrane insertion,” J. Biol. Chem. 290, 19334-19342 (2015).

G. Mitchell, L. Ge, Q. Huang, C. Chen, S. Kianian, M. F. Roberts, R. Schekman, and D. A. Portnoy, “Avoidance of autophagy mediated by PlcA or ActA is required for Listeria monocytogenes growth in macrophages,” Infect.  Immun. 83, 2175-2184 (2015).

Y. Wang, B. Stec, A.G. Redfield, E. Weerapana, and M.F. Roberts, “Phospholipid binding sites of PTEN: Exploring the mechanism of PIP₂ activation,” J. Biol. Chem. 290, 1592-1606 (2015).

B. Yang, M. Pu, H. Khan, L. Friedman, N. Reuter, M. F. Roberts, and A. Gershenson, “Quantifying transient interactions between Bacillus phosphatidylinositol-specific phospholipase C and phosphatidylcholine-rich vesicles,” J. Am. Chem. Soc, 137,14-17 (2015).

J.J. Meidenbauer, and M.F. Roberts, “Reduced glucose utilization underlies seizure protection with dietary therapy in epileptic EL mice,” Epilepsy & Behavior 39, 48-54 (2014).

C.S. Gradziel, Y. Wang, B.  Stec, A.G. Redfield, and M.F. Roberts, “Cytotoxic amphiphiles and phosphoinositides bind to two discrete sites on the Akt1 PH domain,” Biochemistry 53, 462-472. (2014)

O.V. Kurnasov, H.-J.D. Luk, M. F. Roberts, and B. Stec, “Structure of the inositol-1-phosphate cytidylyltransferase from Thermotoga maritima,” Acta Cryst. D 69, 1808-1817 (2013).

J. Cheng, R. Goldstein, A. Gershenson, B. Stec, and M.F. Roberts, “The cation-π box is a specific phosphatidylcholine membrane targeting motif,” J. Biol. Chem. 288, 14863-14873 (2013).

C. Grauffel, B. Yang, T. He, M.F. Roberts, A. Gershenson, and N. Reuter, “Cation-π interactions as lipid-specific anchors for phosphatidylinositol-specific phospholipase C,” J. Am. Chem. Soc. 135, 5740-5750 (2013).

J. Cheng, S. Karri, C. Grauffel, N. Reuter, M.F. Roberts, P.L. Wintrode, and A. Gershenson, “Does changing the predicted dynamics of a phospholipase C alter activity and membrane binding?” Biophys. J. 104, 185-195 (2013).

J. Cai, S. Guo, J. W. Lomasney, and M.F. Roberts, “Ca²⁺-independent binding of anionic phospholipids by phospholipase C δ1 EF-hand domain,” J. Biol. Chem. 288, 37277-37288 (2013).

K. Neelon, M.F. Roberts, and B. Stec, “Crystal structure of a trapped catalytic intermediate suggests that forced atomic proximity drives the catalysis of mIPS,” Biophys. J. 101, 2816-2824 (2011).

C.L. Strelko, W. Lu, F.J. Dufort, T.N. Seyfried, T.C. Chiles, J.D. Rabinowitz, and M.F. Roberts, “Itaconic acid is a mammalian metabolite during macrophage activation,” J. Amer. Chem. Soc. 133, 16386-16389 (2011).

J. Cheng, R. Goldstein, B. Stec, A. Gershenson, and M.F. Roberts, “Competition between anion binding and dimerization modulates S. aureus phosphatidylinositol-specific phospholipase C enzymatic activity,” J. Biol. Chem. 287, 40317-40327 (2012).

R. Goldstein, J. Cheng, B. Stec, and M.F. Roberts, “An intramolecular π-cation latch controls substrate access in the phosphatidylinositol-specific phospholipase C from Staphylococcus aureus,” Biochemistry 51, 2579-2587 (2012).

S.L. Haynie, A.S. Hinkle, N.L. Jones, C.A. Martin, P.J. Olsiewski, and M.F. Roberts, “Reflections on the journey: six short stories,” Chemistry Central Journal 5:69 (2011) doi: 10.1186/1752-153X-5-69.

M. Pu, A. Orr, A.G. Redfield, and M.F. Roberts, “Defining specific lipid binding sites for membrane-bound phospholipase C, by ³¹P NMR from 0.005 to 11.7 Tesla,” J. Biol. Chem. 285, 26916-26922 (2010).

D. Cai, L. Ren, H. Zhao, C. Xu, Y. Yu, H. Wang, Y. Lan, M.F. Roberts, M.J. Naughton, Z. Ren, and T. Chiles, “A molecular imprint nanosensor for ultrasensitive detection of proteins,” Nat. Nanotechnol. 5, 597-601 (2010).

Q. Wang, Y. Wei, M.F. Roberts, and G. Krilov, “Understanding the stereospecific interactions of 3-deoxyphosphatidylinositol derivatives with the PTEN phosphatase domain,” J. Molec. Graphics Model.  29, 102-114 (2010).

Z. Li, K.A. Stieglitz, A.L. Shrout, Y. Wei, R.M. Weis, B. Stec, and M.F. Roberts, “Mobile loop mutations in an archaeal inositol monophosphatase: modulating three-metal ion assisted catalysis and lithium inhibition,” Prot. Sci. 19, 309-318 (2009).

M. Pu, J. Feng, A.G. Redfield, and M.F. Roberts, “Enzymology with a spin-labeled phospholipase C: soluble substrate binding by ³¹P NMR from 0.005 to 11.7 Tesla,” Biochemistry 48, 8282-88284 (2009).

M. Pu, M.F. Roberts, and A. Gershenson, “Fluorescence correlation spectroscopy of phosphatidylinositol-specific phospholipase C monitors the interplay of substrate and activator lipid binding,” Biochemistry 48, 6835-6845 (2009).

M. Pu, X. Fang, A. Gershenson, A.G. Redfield, and M.F. Roberts, “Correlation of vesicle binding and phospholipid dynamics with phospholipase C activity: Insights into phosphatidylcholine activation and surface dilution inhibition,” J. Biol. Chem. 284, 16099-16107 (2009).

X. Shi, C. Shao, X. Zhang, C. Zambonelli, A.G. Redfield, J.F. Head, B.A. Seaton, and M.F. Roberts, “Modulation of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C activity by mutations in the putative dimerization interface,” J. Biol. Chem. 284, 15607-15618 (2009).

C.M. Longo, Y. Wei, M.F. Roberts, and S.J. Miller, “Asymmetric syntheses of L,L- and L,D-di-myo-inositol-1,1’-phosphate and heir behavior as stabilizers of enzyme activity at extreme temperatures, Angew. Chem. Int. Ed. 48, 4158-4161 (2009).

M.F. Roberts, U. Mohanty, and A.G. Redfield, “Phospholipid reorientation at the lipid/water interface measured by high resolution field cycling ³¹ P NMR spectroscopy,” Biophys. J. 97, 132-141 (2009).

W. Chen, H. Goldfine, B. Ananthanarayanan, W. Cho, and M.F. Roberts, “Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Kinetic activation and homing in on different interfaces,” Biochemistry 48, 3578-3592 (2009).

V.N. Sivanandam, J. Cai, A.G. Redfield, and M.F. Roberts, “Phosphatidylcholine “wobble” in vesicles assessed by high-resolution ¹³C field cycling NMR spectroscopy,” J. Am. Chem. Soc. 131, 3420-3421 (2009).

Y.K. Wang, W. Chen, D. Blair, M. Pu, Y. Xu, S.J. Miller, A.G. Redfield, T.C. Chiles, and M. F. Roberts, “Insights into the structural specificity of the cytotoxicity of 3-deoxy-phosphatidylinositols,” J. Am. Chem. Soc. 130, 7746-7755 (2008).

S. Guo, X. Zhang, B. A. Seaton, and M. F. Roberts, “Role of helix B residues in interfacial activation of a bacterial PI-PLC,” Biochemistry 47, 4201-4210 (2008).

J.B. Klauda, M.F. Roberts, A.G. Redfield, B.R. Brooks, and R.W. Pastor, “Rotation of lipids in membranes: MD simulation, ³¹P spin-lattice relaxation, and rigid-body dynamics,” Biophys. J. 94, 3074-3083 (2008).

B.F. Bleiman, F.J. Dufort, M.R. Gumina, D. Blair, D.J. Wagner, M.F. Roberts, and T.C. Chiles, “Interleukin-4-mediated protection of primary B lymphocytes from apoptosis via Stat6-dependent regulation of glycolytic metabolism,” J. Immunol. 179, 4953-4957 (2007).

Y.K. Wang, K.A. Stieglitz, M. Bubunenko, D.L. Court, B. Stec, and M.F. Roberts, “The structure of the R184A mutant of the inositol monophosphatase encoded by suhB and implications its functional interactions in E. coli,” J. Biol. Chem. 282, 26989-26996 (2007).

K.A. Stieglitz, M.F. Roberts, W. Li, and B. Stec, “Crystal structure of the tetrameric IMPase (TM1415) from the hyperthermophile Thermotoga maritima,” FEBS J. 274, 2461-2469 (2007).

E. Crouch, B. McDonald, K Smith, M. Roberts, T. Cafarella, B. Seaton, and J. Head, “Critical role of Arg/Lys343 in the species-dependent recognition of phosphatidylinositol by pulmonary surfactant protein D,” Biochemistry 46, 5160-5169 (2007).

D.A. Rodionov, O.V. Kurnasov, B. Stec, Y. Wang, M.F. Roberts, and A. Osterman, “Genomic identification and in vitro reconstitution of a complete biosynthetic pathway for the osmolyte di-myo-inositol phosphate,” Proc. Natl. Acad. Sci. U.S.A. 104, 4279-4284 (2007).

C. Shao, X. Shi, H. Wehbi, C. Zambonelli, J.F. Head, B.A. Seaton, and M.F. Roberts, “Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C,” J. Biol. Chem., 282, 9228-9235 (2007).

A.C. Faber, F.J. Dufort, D. Blair, D. Wagner, M.F. Roberts, and T.C. Chiles, “Inhibition of phosphatidylinositol 3-kinase-mediated glucose metabolism coincides with resveratrol-induced cell cycle arrest in human diffuse large B-cell lymphomas,” Biochem. Biopharm. 29, 1561-1566 (2006).

C.A. Doughty, B.F. Bleiman, D.J. Wagner, F.J. Dufort, J.M. Mataraza, M.F. Roberts, and T.C. Chiles, “Receptor-mediated changes in glucose metabolism in B lymphocytes: role of phosphatidylinositol-3-kinase signaling in the glycolytic control of growth,” Blood 107, 4458-4465 (2006).

Y.K. Wang, A.J. Morgan, K.A. Stieglitz, B. Stec, B. Thompson, S.J. Miller, and M.F. Roberts, “Temperature dependence of the inositol monophosphatase K_m correlates with di-myo-inositol-1,1’-phosphate accumulation in Archaeoglobus fulgidus,” Biochemistry 45, 3307-3314 (2006).

M.F. Roberts, “Inositol in bacteria and archaea,” Subcell. Biochem. 39, 103-133 (2006).

M.F. Roberts, “Characterization of organic compatible solutes of halotolerant and halophilic organisms,” Meth. Microbiol. 35, 615-647 (2006).

K. Neelon, H.J. Schreier, H. Meekins, P.M. Robinson, and M.F. Roberts, “Compatible solute effects on thermostability of glutamine synthetase and aspartate tanscarbamylase from Methanococcus jannaschii,” Biochim. Biophys. Acta 1753, 164-73 (2005).

M.F. Roberts, “Organic compatible solutes of halotolerant and halophilic microorganisms,” Saline Systems 1: 5 (2005).

M. Kobayashi, Z. Gryczynski, J. Malick, J. Feng3 M.F. Roberts, J.R. Lakowicz, and J.W. Lomasney, “Spectroscopic characterization of the EF-hand domain of phospholipase C–δ1: identification of a lipid interacting domain,” Arch. Biochem. Biophys 440, 191-203 (2005).

K. Neelon, Y. Wang, B. Stec, and M.F. Roberts, “Probing the mechanism of Archaeoglobus fulgidus inositol-1-phosphate synthase,” J. Biol. Chem. 280, 11475-11482 (2005).

J. Feng, M.F. Roberts, G. Drin, and S. Scarlata, “Dissection of the steps of phospholipase Cβ2 activity that are enhanced by Gβγ subunits,” Biochemistry 44, 2577-2584 (2005).

K. Stieglitz, H. Yang, M.F. Roberts, and B. Stec, “Reaching for mechanistic consensus across kingdoms: structure and insights into catalysis of the inositol-1-phosphate synthase (mIPS) from Archaeoglobus fulgidus,” Biochemistry 44, 213-224 (2005).

C. Zambonelli and M.F. Roberts, “Non-HKD phospholipase D enzymes: new players in phosphatidic acid signaling?” Progr. Nucl. Acid Res. Molec. Biol.,79, 133-181 (2005).